FIGURE

Fig. 4.

ID

ZDB-FIG-190723-880

Publication

Gjini et al., 2019 - Disruption of asxl1 results in myeloproliferative neoplasms in zebrafish

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Fig. 4.

Complete loss of asxl1 leads to AML in zebrafish. WISH for cmyb was performed at 3 dpf in asxl1^+/+ (A), asxl1^+/⁻ (B) and asxl1^−/− (C) zebrafish embryos obtained from a cross of two asxl1^+/⁻ fish. (D) WISH for cmyb was performed at 3 dpf in asxl1^−/− zebrafish embryos obtained from a cross of two asxl1^−/− fish. Insets show a higher magnification of the CHT in each panel. (E) MGG staining was performed on kidney marrow smears and peripheral blood smears of asxl1^+/+ and asxl1^−/− fish at 17 months of age. Normal maturation and morphology are shown across a spread of blood cells in the kidney marrow of the asxl1^+/+ zebrafish. In the asxl1^−/− fish, the kidney marrow is replaced with immature myeloid blast cells in a pattern resembling AML. Circulating immature myeloid blast cells were observed in the peripheral blood of the asxl1^−/− zebrafish, but not asxl1^+/+ fish. Erythrocytes, black arrow; myeloid cells, green arrow; blast cells, blue arrow; lymphocytes, red arrow.

Expression Data

Gene:	myb
Fish:	WT asxl1^zdf34/zdf34 asxl1^zdf34/+
Anatomical Term:	caudal hematopoietic tissue hematopoietic multipotent progenitor cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	asxl1^zdf34/zdf34 asxl1^zdf34/zdf34 asxl1^zdf35/zdf35
Observed In:	blood myeloblast caudal hematopoietic tissue hematopoietic multipotent progenitor cell head kidney myeloblast hemopoiesis
Stage Range:	Protruding-mouth to Adult

Phenotype Detail

Acknowledgments

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