Fig. 1

Generating the putative Q₅₂, Q₃₁ and Q₁₀-GFP constructs using the Tol2-Q₈₀-GFP-v2A-GFP multicistronic reporter construct. a Vector map of the Tol2-Q₈₀-GFP-v2A-GFP construct. b Summary of CAA and CAG degenerate codon usage in this construct. c Chromatograph of the construct depicting the randomly interspaced glutamine coding CAG and CAA triplets. d Schematic illustration of PCR-based exclusion amplification of the Q₈₀-GFP containing construct to generate polyQ constructs with lower numbers of glutamine repeats. e Q₈₀ sequence showing Q₅₂, Q₃₁ and Q₁₀ primer binding sites. f Sequences of primers intended to generate Q₅₂, Q₃₁ and Q₁₀ constructs. g Analytical agarose gel electrophoresis for each of the putative Q₈₀, Q₅₂, Q₃₁ and Q₁₀ vectors using primers flanking the polyQ region. PCR product sizes of ~ 270, ~ 180, ~ 120 and ~ 60 bp for the intended putative Q₈₀, Q₅₂, Q₃₁ and Q₁₀-GFP constructs, respectively, were seen