Fig. 3

RBFOX1 RRM interaction with RNAs containing consecutive binding sites using switchSENSE. a Schematic view of hybridization of the DNA/RNA chimeric molecule to the ssDNA covalently attached to the biochip electrodes. The sequences of ssDNA and DNA/RNA molecules are shown. b Sizing experiments performed with 150 nM of RBFOX1 RRM in T40 buffer at 37 °C. Results are shown for the RBFOX1×4 and SCRM×4 DNA/RNA molecules (left side) and a graph shows the apparent size measured with RNA containing one (×1), two (×2), three (×3), and four (×4) consecutive RBFOX1 binding sites (right side). c Titrations performed using switchSENSE on three different electrodes (red, blue and green colors) with increasing concentrations (0.3, 0.6, 1.2, 2.3, 4.7, 9.4, 18.8, 37.5, 75, 150 nM) of RBFOX1 RRM and ×1, ×2, ×3, and ×4 DNA/RNA molecules. Experiments were performed at 37 °C in T100 buffer. K_D values obtained from global Langmuir fits are indicated for each interaction with the corresponding standard deviation