FIGURE

Fig 1

ID
ZDB-FIG-190723-1207
Publication
Feng et al., 2016 - Correction: Live Imaging of Innate Immune Cell Sensing of Transformed Cells in Zebrafish Larvae: Parallels between Tumor Initiation and Wound Inflammation
Other Figures
All Figure Page
Back to All Figure Page
Fig 1

Activation of leukocytes in zebrafish larvae by oncogene transformed cells.

(A) Schematic of the procedure for transient induction of V12RAS/v-Src in embryos that also have fluorescently-tagged neutrophils, with an example of a V12RAS+ melanoblast clone (red) in a 3dpf larva also expressing eGFP (green) in neutrophils. (B) (i) A 5dpf MPO:GFP larva with a V12RAS+ clone (red). (ii) High magnification view of the inset in (B) (i), which is a single image from a time-lapse movie (Video S1A) of GFP-tagged neutrophils actively interacting with a V12RAS+ clone; note that most of the red fluorescent signal is quenched by melanocyte pigment. (C) (i) A single image from a time-lapse movie showing LysC:DsRed+ cells recruited v-Src+ (green) cells in a 3dpf larva (Video S2B) (ii) An equivalent image from a time-lapse movie showing no recruitment of LysC:DsRed+ cells to GAP43-eGFP expressing cells in a control larva (Video S2A). (D) (i) Low magnification, two-channel, lateral view of a control Tg (kita:GalTA4; UAS:eGFP; LysC:DsRed) larva at 4dpf. (ii) Single channel view to highlight only the DsRed-tagged leukocytes. The box highlights these cells located within the caudal hematopoietic tissue. (E) As for D but of a Tg (kita:GalTA4; UAS:V12RASeGFP; LysC:DsRed) larva at 4 dpf. The boxed zone in E (ii) indicates how the LysC:DsRed+ cells have largely dispersed from the caudal hematopoietic tissue into the flank skin. (F) A confocal Z-stack projection of the flank of a control larva in the trunk region; (G) Equivalent image to E but of a V12RAS+ larva; both are stained with the anti-L-plastin antibody (magenta). (H) A high magnification view of a larva similar to that in (F), illustrating the association of L-plastin+ cells (magenta) with V12RAS+ cells (green). (I) A similar larvae to that in H, but with v-Src expressing cells (green) (J) Anti-BrdU (red) immunostaining of control mucus secreting cells (green). (K) Anti-BrdU (red) immunostaining of V12RAS+ mucus secreting cells (green). (L) Quantification of numbers of LysC:DsRed+ cells present in the skin of the trunk in the region indicated by boxes in (D) and (E). (M) Quantification of the number of L-plastin+ cells present in the trunk epidermis in regions indicated in (F) and (G). *** p<0.001; Larval images in (D) and (E) have been “tiled” together from several micrographs and the tiling borders are indicated with white dotted lines. Scale bars: (A) = 48 μm; (B) = 24 μm; (C) = 20 μm; (D, E, F, G) = 150 μm; (I, J, K) = 16 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.