FIGURE

Fig. 5

ID

ZDB-FIG-190716-49

Publication

Ando et al., 2019 - Cereblon Control of Zebrafish Brain Size by Regulation of Neural Stem Cell Proliferation

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Sox2 Expression in crbn-Overexpressing Embryos

(A) Embryos left uninjected or injected with mRNA encoding gfp, crbn^ΔMid, or crbn^WT were immunostained using anti-Sox2 antibody and counterstained with DAPI at 9 hpf. Bright-field (upper panels) and fluorescence (lower panels) images are shown. Animal pole to the top, dorsal side to the right. Dorsal epidermis (the early brain field) is indicated with asterisks.

(B) Illustration of 9-hpf embryos. Dorsal epidermis is indicated with dashed line. Lower panels in (C) correspond to the area indicated with red rectangle.

(D) Quantification and statistical analysis of the data shown in (C). DAPI-positive cells and Sox2-positive cells in the early brain field of 9-hpf embryos were counted and are shown as means ± SD (n = 15 per group).

Scale bar, 150 μm. ***p < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ iScience