FIGURE

Fig. 5

ID

ZDB-FIG-190618-28

Publication

Park et al., 2019 - 3-ketodihydrosphingosine reductase mutation induces steatosis and hepatic injury in zebrafish

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Fig. 5

Sphk2 deletion suppresses liver defects in the kdsr^I105R mutant. Images of live wild type (wt), kdsr^I105R, and kdsr^I105R; sphk2^wc1 double mutant at 7 dpf (A). Scale bar = 0.25 mm. Relative mRNA expression of sphk2 and other genes associated with inflammation (tnfa, il1b), tissue injury (alpha1-anti-trypsin (a1at)) and fibrosis (col1a1a) (B), sphingolipid salvage pathway related genes (C) in wt, kdsr^I105R, sphk2^wc1, and kdsr^I105R; sphk2^wc1 mutant siblings. spp1, (S1P-phosphohydrolase 1). Images of H&E and ORO staining in livers of wt, kdsr^I105R and kdsr^I105R; sphk2^wc1double mutants (D, n = 6). Error bars indicate standard deviation of the mean. *P ≤ 0.05, **P ≤ 0.005. Scale bare in D = 100 µm.

Expression Data

Genes:	asah1a asah1b asah2 col1a1a il1b serpina1 sgpl1 sgpp1b sphk2 tnfa (all 10)
Fish:	AB/TU kdsr^{muz106/muz106} (AB/TU) sphk2^wcm2/wcm2 (AB/TU) kdsr^{muz106/muz106}; sphk2^wcm2/wcm2 (AB/TU)
Anatomical Term:	whole organism
Stage:	Days 7-13

Expression Detail

Gene	Fish	Conditions	Stage	Anatomy	Assay
asah1a	AB/TU	standard conditions	Days 7-13	whole organism	RTPCR
asah1a	kdsr^{muz106/muz106} (AB/TU)	standard conditions	Days 7-13	whole organism	RTPCR
asah1a	kdsr^{muz106/muz106}; sphk2^wcm2/wcm2 (AB/TU)	standard conditions	Days 7-13	whole organism	RTPCR
asah1a	sphk2^wcm2/wcm2 (AB/TU)	standard conditions	Days 7-13	whole organism	RTPCR
asah1b	AB/TU	standard conditions	Days 7-13	whole organism	RTPCR
asah1b	kdsr^{muz106/muz106} (AB/TU)	standard conditions	Days 7-13	whole organism	RTPCR
asah1b	kdsr^{muz106/muz106}; sphk2^wcm2/wcm2 (AB/TU)	standard conditions	Days 7-13	whole organism	RTPCR
asah1b	sphk2^wcm2/wcm2 (AB/TU)	standard conditions	Days 7-13	whole organism	RTPCR
asah2	AB/TU	standard conditions	Days 7-13	whole organism	RTPCR
asah2	kdsr^{muz106/muz106} (AB/TU)	standard conditions	Days 7-13	whole organism	RTPCR

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Antibody Labeling

Phenotype Data

Fish:	kdsr^{muz106/muz106} (AB/TU) sphk2^wcm2/wcm2 (AB/TU) kdsr^{muz106/muz106}; sphk2^wcm2/wcm2 (AB/TU)
Observed In:	hepatocyte liver liver lipid whole organism
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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