Fig. 6

 Analysis of maternal-zygote mutant and heterozygote first pharyngeal arch defects in fixed whole mount specimens and in specimens stained using Alcian Blue and Alizarin Red dyes.

(A) Maternal mutant heterozygote larvae, 72 hpf, lateral view. (B) Maternal-zygotic mutant, 72 hpf, lateral view. Arrow indicates facial reduction. (C) Maternal-zygotic mutant pharyngeal skeleton showing loss of first pharyngeal arch elements (see Figs. 2A and 2D for reference), 7 dpf, lateral view. (D and E) Neurocrania of maternal mutant-zygotic heterozygote larvae showing normal patterning (D) and defects (E), 7 dpf, ventral views. (F) Neurocranium of maternal-zygotic mutant, 7 dpf, ventral view. (G) Total scores of maternal-zygotic mutants and heterozygotes for first pharyngeal arch skeletal defects and neurocranium defects. Abbreviations: en, entopterygoid; m, Meckel’s cartilage; pq, palatoquadrate; q, quadrate. Scale bar, 200 microns. Cartilage is stained blue (Alcian Blue), bone is stained red (Alizarin Red)