FIGURE
Fig. 5
- ID
- ZDB-FIG-181207-4
- Publication
- Endlich et al., 2018 - BDNF: mRNA expression in urine cells of patients with chronic kidney disease and its role in kidney function
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Fig. 5
Bdnf knockdown in zebrafish larvae leads to pericardial oedema formation, impaired glomerular filtration and down‐regulation of podocyte‐specific genes. Bdnf morpholinos (bdnfMO) and control morpholinos (CtrlMO) were injected in ET zebrafish eggs. Brightfield pictures were taken 3 dpf (A; a and b) and 6 dpf (A; e and f). bdnfMO‐injected larvae exhibit a higher rate of pericardial oedema development and a lower viability than normal controls (B). Morpholino injection in CADE larvae reveals an impairment of glomerular filtration barrier function in bdnfMO‐injected larvae 3 dpf (A; c) and 6 dpf (A; g) compared to CtrlMO‐injected larvae (A; d and f). The knockdown of bdnf was verified by RT‐PCR (C). The down‐regulation of the podocyte marker nphs2 was verified on the mRNA level by RT‐PCR (C) and qRT‐PCR (D). Expression levels were normalized to zgc:158463 in RT‐PCR and to zgc:158463 and eef1a1/1 in qRT‐PCR by the ΔΔCt method. [Scale bars = 500 μm] [*P < .05, ***P < .001]
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Expression Data
| Genes: | |
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| Fish: | |
| Knockdown Reagent: | |
| Anatomical Terms: | |
| Stage Range: | Protruding-mouth to Day 6 |
Expression Detail
Antibody Labeling
Phenotype Data
| Fish: | |
|---|---|
| Knockdown Reagent: | |
| Observed In: | |
| Stage Range: | Protruding-mouth to Day 6 |
Phenotype Detail
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Full text @ J. Cell. Mol. Med.