ZFIN Figure: Mansour et al., 2018, Fig. 7

ZFIN ID: ZDB-FIG-181030-9

ZFIN ID: ZDB-FIG-181030-9

Mansour et al., 2018 - JDP2: An oncogenic bZIP transcription factor in T cell acute lymphoblastic leukemia. The Journal of experimental medicine 215(7):1929-1945 Full text @ J. Exp. Med.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	EGFP mCherry
Fish:	zdf6Tg zf2187Tg zdf6Tg; zf2187Tg
Condition:	chemical treatment by environment: dexamethasone
Anatomical Term:	thymus
Stage:	Days 7-13

PHENOTYPE:

Fish:	zdf6Tg zf2187Tg zdf6Tg; zf2187Tg
Condition:	chemical treatment by environment: dexamethasone
Observed In:	thymus
Stage:	Days 7-13

Fig. 7

Thymuses of Tg(rag2:jdp2) fish are resistant to dexamethasone. (A) Representative fluorescent images of thymuses from 8-d-old stable transgenic zebrafish larvae exposed to dexamethasone (25 µg/ml) or DMSO control, from day 5 postfertilization (dpf). Bar, 0.1 mm. (B) Quantification of thymic fluorescence in 8-d-old stable transgenic zebrafish larvae exposed to 25 µg/ml dexamethasone or DMSO control from 5 dpf. Values are expressed as a percentage of thymic fluorescence in DMSO control treated zebrafish, and P values were calculated using two-tailed Student’s t test. Findings were validated in three independent experiments.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
EGFP	zdf6Tg	chemical treatment by environment: dexamethasone	Days 7-13	thymus	IFL
mCherry	zdf6Tg; zf2187Tg	chemical treatment by environment: dexamethasone	Days 7-13	thymus	IFL
	zf2187Tg	chemical treatment by environment: dexamethasone	Days 7-13	thymus	IFL

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
zdf6Tg	chemical treatment by environment: dexamethasone	Days 7-13	thymus EGFP expression decreased amount, abnormal
zdf6Tg; zf2187Tg	chemical treatment by environment: dexamethasone	Days 7-13	thymus mCherry expression amount, ameliorated
zf2187Tg	chemical treatment by environment: dexamethasone	Days 7-13	thymus mCherry expression amount, ameliorated

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Exp. Med.