FIGURE

Fig. 1

ID

ZDB-FIG-180828-10

Publication

Zhang et al., 2018 - Apoptosis of Endothelial Cells Contributes to Brain Vessel Pruning of Zebrafish During Development

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Fig. 1

Vessel pruning is accompanied with endothelial cell (EC) apoptosis in the brain of larval zebrafish. (A) In vivo time-lapse confocal images showing that an EC (arrows) underwent apoptosis on a pruned brain vessel in a Tg(fli1a:nEGFP);Tg(fli1a.ep:DsRedEx) larva at 3–3.5 days post-fertilization (dpf), in which EC nuclei were labeled by both enhanced green fluorescent protein (EGFP) and DsRed (yellow). Left, projected confocal image of the whole brain vasculature; Right, time-lapse confocal images of the dashed outlined area in the left. (B) Summary of the percentages of EC apoptosis- and migration-accompanied brain vessel pruning (n = 17 larvae). (C) In vivo time-lapse confocal images showing that an EC (arrows) underwent apoptosis on a brain pruned vessel in a DAPI-injected Tg(kdrl:EGFP) larva at 3–3.5 dpf, in which EC nuclei were labeled by DAPI (blue). (D) Immunofluorescence images showing that, on a pruned vessel, an EC with typical apoptotic morphology (arrows) expressed Caspase-3. The numbers on the bars (B) represent the number of pruned vessels examined. Scales: 50 μm (left in (A), 15 μm (right in (A), 50 μm (C) and 15 μm (D).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.