ZFIN ID: ZDB-FIG-180820-9
Zhong et al., 2018 - Ezh2 promotes clock function and hematopoiesis independent of histone methyltransferase activity in zebrafish. Nucleic acids research   46(7):3382-3399 Full text @ Nucleic Acids Res.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Day 4

Fig. 8

Definitive hematopoiesis is disrupted in ezh2–/– mutant zebrafish. (A–L) In situ hybridization of WT sibling (A, C, E, G, I and K) and ezh2–/– mutant embryos or larvae (B, D, F, G, J and L) using probes of runx1 (A, B), cmyb (C, D), ikaros (E, H), lck (I, J), rag-1 (K, L) at 28 hpf (A, F) and 96 hpf (G, L), respectively. White arrowheads indicate ICM in A, C and E and black arrowheads the thymus in G, I and K. Scale bar, 0.25 mm. (M–P) Images of cmyb:eGFP cells in wild-type (M, O) and ezh2–/– mutant (N, P) fish at 32 hpf (M, N) and 72 hpf (O, P). (Q–T) Images of coro1a:eGFP cells in wild-type (Q, S) and ezh2–/– mutant (R, T) fish at 28 hpf (Q, R) and 72 hpf (S, T). White arrowheads indicate ICM in M, N, Q and R and dashed circle the thymus in O, P, S and T. Scale bar in (M–T), 0.25 mm. All images shown are lateral view, anterior to left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP ezh2sa1199/sa1199; hkz04tTg control Prim-5 intermediate cell mass of mesoderm IFL
Protruding-mouth Not Detected thymus IFL
ezh2sa1199/sa1199; zf169Tg control Prim-15 intermediate cell mass of mesoderm IFL
Protruding-mouth Not Detected thymus IFL
hkz04tTg control Prim-5 intermediate cell mass of mesoderm IFL
Protruding-mouth thymus IFL
zf169Tg control Prim-15 intermediate cell mass of mesoderm IFL
Protruding-mouth thymus IFL
ikzf1 WT control Prim-5 ventral wall of dorsal aorta ISH
Day 4 thymus ISH
ezh2sa1199/sa1199 control Prim-5 ventral wall of dorsal aorta ISH
Day 4 Not Detected thymus ISH
lck WT control Day 4 thymus ISH
ezh2sa1199/sa1199 control Day 4 Not Detected thymus ISH
myb WT control Prim-5 ventral wall of dorsal aorta ISH
ezh2sa1199/sa1199 control Prim-5 ventral wall of dorsal aorta ISH
rag1 WT control Day 4 thymus ISH
ezh2sa1199/sa1199 control Day 4 Not Detected thymus ISH
runx1 WT control Prim-5 ventral wall of dorsal aorta ISH
ezh2sa1199/sa1199 control Prim-5 ventral wall of dorsal aorta ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ezh2sa1199/sa1199 control Prim-5 hematopoietic stem cell differentiation decreased occurrence, abnormal
Prim-5 ventral wall of dorsal aorta ikzf1 expression decreased amount, abnormal
Prim-5 ventral wall of dorsal aorta myb expression decreased amount, abnormal
Prim-5 ventral wall of dorsal aorta runx1 expression decreased amount, abnormal
Day 4 definitive hemopoiesis decreased occurrence, abnormal
Day 4 thymus ikzf1 expression absent, abnormal
Day 4 thymus lck expression absent, abnormal
Day 4 thymus rag1 expression absent, abnormal
ezh2sa1199/sa1199; hkz04tTg control Prim-5 hematopoietic stem cell differentiation decreased occurrence, abnormal
Prim-5 intermediate cell mass of mesoderm EGFP expression decreased distribution, abnormal
Protruding-mouth definitive hemopoiesis decreased occurrence, abnormal
Protruding-mouth definitive hemopoiesis decreased occurrence, abnormal
Protruding-mouth thymus EGFP expression absent, abnormal
ezh2sa1199/sa1199; zf169Tg control Prim-15 definitive hemopoiesis decreased occurrence, abnormal
Prim-15 intermediate cell mass of mesoderm EGFP expression decreased distribution, abnormal
Protruding-mouth definitive hemopoiesis decreased occurrence, abnormal
Protruding-mouth definitive hemopoiesis decreased occurrence, abnormal
Protruding-mouth thymus EGFP expression absent, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.