Fig. 7
- ID
- ZDB-FIG-180807-14
- Publication
- Lai et al., 2018 - Liver-directed microRNA-7a depletion induces nonalcoholic fatty liver disease by stabilizing YY1-mediated lipogenic pathways in zebrafish
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Inhibition of YY1 expression rescues the NAFLD phenotypes in hC7aSP1 larvae. (A) Steatotic analyses of 9 dpf WT and hC7aSP1 larvae. Embryos without injection (NI) and those injected with 4 ng ASO-miR-7a, 200 pg YY1 mRNA, 100 pg YY1 mRNA + 2 ng ASO-miR-7a, 6 ng miR-7a-mimic, and 100 pg YY1 mRNA + 3 ng miR-7a-mimic were analyzed at the embryonic stage of 7 dpf. (B) Quantification of hepatic steatosis of WT and hC7aSP1 larvae in (A) fed with LFD. Percentages of each category in the WT and hC7aSP1 larvae in (A) are presented. The total number (N) of larvae in each group is indicated (n = 40–50 for each groups). *p < 0.01, and **p < 0.005. (C) Western blot analyses of YY1 depletive efficiency. WT and hC7aSP1 embryos without injection (NI) and those injected with 8 ng miR-7a-mimic were harvested at 9 dpf. β-Actin was used as the loading control. |