FIGURE

Fig. 5

ID

ZDB-FIG-180801-29

Publication

LLeras Forero et al., 2018 - Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock

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Fig. 5

Inaccurate spacing of entpd5+ segments results in erroneous chordacentrum formation.

(A, B) Time series images of entpd5+ segments around the notochord in her1;her7 mutants, in lateral view with anterior to the left. (A) An atypically wide space between entpd5+ segments (arrow) results in the subsequent intercalation of an additional, smaller entpd5+ segment (1´). (B) An additional smaller segment (2') fuses to adjacent vertebra. (C) The length between existing entpd5+ segments was measured in her1;her7 mutants (n = 4) in positions where an entpd5 ring would be intercalated (red dots) and compared to the equivalent axial position in wild type (WT, n = 16) (black crosses). The distance preceding an intercalation in her1;her7 mutants was either similar or larger than wild type. DM, distance measured; E entpd5+ segment. All scale bars are 100 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	her7^{hu2526/hu2526}; her1^{zf2173/zf2173}; hu6867Tg
Observed In:	centrum
Stage Range:	Days 14-20 to Days 21-29

Phenotype Detail

Acknowledgments

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