ZFIN ID: ZDB-FIG-180723-19
Fassier et al., 2018 - Motor axon navigation relies on Fidgetin-like 1-driven microtubule plus end dynamics. The Journal of cell biology   217(5):1719-1738 Full text @ J. Cell Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage: Prim-5

Fig. 2

Fignl1 associates with MTs in spinal motor axons. (A) Immunodetection of Fignl1 (red), acetylated α-tubulin (blue), and GFP (green) in 26-hpf Tg(Hb9:GFP) transgenic embryos. Lateral views of the trunk. (B) GC from cultured Tg(Hb9:GFP) SMN labeled with phalloidin (green), tyrosinated tubulin (blue), and Fignl1 (red) antibodies. Arrows indicate actin protrusions. (C) Detergent extraction of soluble proteins from WT or Tg(Hb9:GFP) spinal neurons immunostained by tyrosinated tubulin (red) and Fignl1 or GFP (green). Bars: (A and B) 10 µm; (C) 20 µm. (D) MT copelleting assay from COS-7 cells transfected with pCS2+-HA or pCS2+-HA-Fignl1 (HA-Fignl1). Supernatants (S) were incubated with taxol and GTP to induce MT polymerization and centrifuged on a sucrose cushion to pellet MTs and their associated proteins (P2). Soluble tubulins and proteins that did not bind MTs remained in the S2 supernatant. WBs assessed Fignl1 and α-tubulin levels in different fractions. L, total lysate.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fignl1 Ab1-fignl1 ml2Tg control Prim-5 secondary motor neuron axon IHC
Ab1-fignl1 Prim-5 secondary motor neuron microtubule IHC
GFP ml2Tg control Prim-5 secondary motor neuron axon IHC
Prim-5 secondary motor neuron cell body IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-tuba IHC ml2Tg control Prim-5 secondary motor neuron axon
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Biol.