FIGURE

Fig. 8

ID

ZDB-FIG-180713-28

Publication

Lal et al., 2018 - Identification of a neuronal population in the telencephalon essential for fear conditioning in zebrafish

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Fig. 8

Immunohistochemical analyses of the 120A-Dm neurons. a Fluorescence imaging of the brain of SAGFF120A;UAS:GFP fish from the dorsal side. Dm, medial zone of the dorsal telencephalic area. Scale bars, 500 μm. b–d Double immunofluorescence staining of the telencephalon of SAGFF120A;UAS:GFP fish using the anti-GFP (green) (b) and anti-NeuN (magenta; neuronal marker) (c) antibodies. d A merged image; 16% (1986/12282) of NeuN-positive cells were GFP-positive and 99% (2394/2423) of GFP-positive cells were NeuN-positive. e A coronal view of the zebrafish telencephalon. Dm, medial zone of dorsal telencephalic area (D); Dl, lateral zone of D; Dc, central zone of D; Dp, posterior zone of D; SY, sulcus ypsiloniformis; Vd, dorsal nucleus of ventral telencephalic area (V); Vv, ventral nucleus of V; EN, entopeduncular nucleus. f Fluorescence in situ hybridization using the vglut1/2.1/2.2 probes. g Fluorescence in situ hybridization using the GAD67 probe. h–j Immunofluorescence staining of the telencephalon of SAGFF120A;UAS:GFP fish using anti-GFP (green) (h) and fluorescence in situ hybridization using vglut1/2.1/2.2 probes (magenta) (i). j A merged image; 94% (352/374) of GFP-positive cells were glutamatergic. Scale bars, 200 μm (f, g) and 50 μm (b–d, h–j)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Biol.