Fig. 3

Gal4FF transgenic fish lines that showed deficits in the active avoidance response and had expression patterns in the Dm. a–f Performance of two-way active avoidance response of wild type fish (n = 28) (Fig. 2 and shown in dotted lines) and double transgenic fish that are created by crossing the Gal4FF transgenic fish with the UAS:zBoTxBLC:GFP fish. a SAGFF36B (n = 10). (b) SAGFF70A (n = 22). (c) SAGFF120A (n = 10). d SAGFF228A (n = 10). e SAGFF231A (n = 13). f SAGFF234D (n = 11). Means ± SEM and avoidance (%) for individual fish are plotted. g Comparison of performance of avoidance responses at day 5 with Tukey box plot. Mean is marked by ‘+’. Two-way ANOVA, fish groups (CS-US wild type, CS only wild type described in Fig. 2c, d and all double transgenic fish including fish described in Additional file 2: Figure S1) x training days (day 1, day 5), was performed (F = 7.236, P < 0.0001). Dunnett’s multiple comparison post-hoc tests were performed between wild type and double transgenic fish on day 5. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. ns, not significant (P > 0.05). h A coronal view of the zebrafish telencephalon. Dm, medial zone of dorsal telencephalic area (D); Dl, lateral zone of D; Dc, central zone of D; Dp, posterior zone of D; SY, sulcus ypsiloniformis; Vd, dorsal nucleus of ventral telencephalic area (V); Vv, ventral nucleus of V; EN, entopeduncular nucleus. i–n GFP expression patterns in the coronal section of the Gal4FF;UAS:GFP transgenic fish with magnified views of Dm. The Gal4FF transgenic fish are crossed with UAS:GFP effector fish. i SAGFF36B. j SAGFF70A. k SAGFF120A. l SAGFF228A. m SAGFF231A. n SAGFF234D. Scale bars, 200 μm