FIGURE

Fig. 4

ID

ZDB-FIG-180713-11

Publication

Zhang et al., 2018 - The DEAD box RNA helicase Ddx39ab is essential for myocyte and lens development in zebrafish

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Fig. 4

Lens fiber cell differentiation is defective in ddx39ab mutant embryos. (A) ddx39ab mutants exhibit defects in lens fiber morphogenesis. (Top) Bright-field images of the eyes from wild-type and ddx39ab mutant zebrafish embryos at 28 hpf. The scatter plot shows the diameter of individual lens fibers. (Middle) Equatorial sections through the lens center showing the organization of lens fibers; cell membrane is labeled with mRFP. Cell number was quantified on equatorial sections. (Bottom) Schematics showing the shape and arrangement of lens fiber cells. The convexity of lens fiber cells is plotted. Scale bars: 50 μm. (B,C) RNA in situ hybridization and qPCR results for lens gene expression in wild-type and ddx39ab mutant zebrafish embryos at 32 hpf. Frontal views with dorsal side to the top. (A-C) At least 20 embryos of each genotype were analyzed and representative samples are shown. All data are mean±s.e.m. n.s, not significant. **P<0.01, ***P<0.001.

Expression Data

Genes:	bfsp1 bfsp2 cryaa crygm2d1 crygm2d10 foxe3 lim2.4 pitx3 prox1a
Fish:	WT ddx39ab^{nju203Gt/nju203Gt}
Anatomical Terms:	lens whole organism
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ddx39ab^{nju203Gt/nju203Gt}
Observed In:	lens lens fiber cell whole organism
Stage Range:	Prim-5 to Prim-15

Phenotype Detail

Acknowledgments

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