FIGURE

Fig. 4

ID

ZDB-FIG-180711-28

Publication

Selland et al., 2018 - Coordinate regulation of retinoic acid synthesis by pbx genes and fibroblast growth factor signaling by hoxb1b is required for hindbrain patterning and development

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Fig. 4

Hoxb1b^−/−;pbx4^−/− double mutants revert to the r1-like ground state.

Eng2 expression in the midbrain-hindbrain boundary is observed in all embryos (A-D), however krox20 and epha4a expression are altered. As previously observed, hoxb1b^−/− mutants have an expanded r3 domain and a reduced r4 domain (6/6 embryos) (B, F). In pbx4^−/− mutants r3 is drastically reduced (12/12 embryos)(C,G), and in hoxb1b^−/−;pbx4^−/− double mutants all krox20 expression in r3 and r5 is lost (3/3 embryos)(D), while the r1 domain of epha4a expression is expanded posteriorly throughout the hindbrain (3/3 embryos)(H). All embryos have been genotyped for pbx4^b557 and hoxb1b^ua1006. All images are dorsal views, anterior to the left, 22hpf.

Expression Data

Genes:	egr2b en2a epha4a
Fish:	AB hoxb1b^{ua1006/ua1006} pbx4^b557/b557 pbx4^b557/b557; hoxb1b^{ua1006/ua1006}
Anatomical Terms:	midbrain hindbrain boundary rhombomere 1 rhombomere 3 rhombomere 5
Stage:	26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hoxb1b^{ua1006/ua1006} pbx4^b557/b557 pbx4^b557/b557; hoxb1b^{ua1006/ua1006}
Observed In:	rhombomere 1 rhombomere 3 rhombomere 4 rhombomere 5
Stage:	26+ somites

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 150, Selland, L.G., Koch, S., Laraque, M., Waskiewicz, A.J., Coordinate regulation of retinoic acid synthesis by pbx genes and fibroblast growth factor signaling by hoxb1b is required for hindbrain patterning and development, 28-41, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.