Fig. 5

Dopaminergic innervation of the posterior lateral line neuromasts. (A–D) Panels 1: schematic showing location of projections (green) and neuromasts (circles) relative to anatomical landmarks. Panels 2 and 5 (green): th:Gal4 driven eGFP-CAAX and SypGFP (GFP) expression visualized by immunofluorescene. Panels 3 and 5 (magenta): anti-TH immunofluorescene. Panels 4 and 5 (blue): TOTO-3 labeling of cell nuclei. MIP of confocal stacks, step size (A–D) 1 μm, scale bars: 50 μm. Arrowheads point at neuromasts. (A). Left lateral part of head dorsal view of DA projections to NM of the medial branch (MI) and dorsal NM D2, as well as projection going through the posterior lateral line ganglion (PG) and toward the posterior lateral line nerve. Total depth of 175 μm. (B). Dorsal view of DA projection to NM L1 and LII.1. Total depth of 130 μm. (C). Dorsal view of DA projection to neuromasts of the dorsal branch (D) of the posterior lateral line. Asterisk indicates putatively developing neuromast without innervation by GFP labeled fibers. Total depth of 60 μm. (D). Lateral view of DA projection to tail NM of the lateral branch (L) of the posterior lateral line. Total depth of 25 μm.