ZFIN ID: ZDB-FIG-180620-39
Leacock et al., 2018 - Structure/Function Studies of the α4 Subunit Reveal Evolutionary Loss of a GlyR Subtype Involved in Startle and Escape Responses. Frontiers in molecular neuroscience   11:23 Full text @ Front. Mol. Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth

Fig. 7

A novel zebrafish glra4a gene trap reveals expression in the zebrafish brain and spinal cord. (A) The zebrafish SAIGFF16B line contains the gene trap construct T2KSAGFF inserted between exons 1 and 2 of glra4a. This cassette contains a splice acceptor (SA), followed by an internal ribosome entry site (IRES), the coding region for the Gal4FF transcription activator and a polyadenylation site (pA). Gal4FF expression in SAIGFF16B was visualized by creating double transgenic fish carrying the Gal4FF transgene and a GFP reporter gene placed downstream of the Gal4-recognition sequence (UAS:GFP). (B,C) GFP expression in the glra4a gene-trap line at 48 hours post fertilization (hpf) reveals that glra4a is predominantly expressed four clusters of hindbrain commissural neurons and selected spinal commissural interneurons. (D) High magnification dorsal view of neurons indicated by arrows in (C). (E,F) Images show a portion of the zebrafish spinal cord at 48 hpf (E) and 72 hpf (F) showing glra4a expression in commissural primary (CoPA, white arrows) and secondary (CoSA, orange arrows). Scale bars: (B): 500 μm; (C); 250 μm; (D): 150 μm; (E,F): 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nksaigff16bGt; nkuasgfp1aTg standard conditions Long-pec brainstem and spinal white matter interneuron IFL
Long-pec hindbrain commissure neuron IFL
Protruding-mouth CoPA IFL
Protruding-mouth CoSA IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.