ZFIN ID: ZDB-FIG-180608-106
Barske et al., 2018 - Essential Role of Nr2f Nuclear Receptors in Patterning the Vertebrate Upper Jaw. Developmental Cell   44(3):337-347.e5 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Prim-25

Fig. 4

Nr2f2/5 Broadly Inhibit Mandibular Gene Expression

(A) Venn diagrams depicting the overlap between genes inhibited and activated by Nr2fs and Edn1.

(B) Hierarchical clustering of log2 fold-change values relative to controls for 350 arch-enriched genes in nr2f2; nr2f5 mutants, sox10:Gal4VP16; UAS:Nr2f5 transgenics (Nr2f5 OE), edn1 mutants, and hsp70l:Gal4; UAS:Edn1 (Edn1 OE) transgenics. Reference data are from Askary et al. (2017). See Table S1 for transcripts per million (TPM) values of all arch-enriched genes.

(C and D) Fluorescence in situ images for dysregulated genes (magenta) relative to all arch NCCs (dlx2a+, green) in nr2f2; nr2f5 mutants (nr2f1b genotype indicated) and sox10:Gal4VP16; UAS:Nr2f5 transgenics at 24 (C) or 36 hpf (D). Arrowheads indicate ectopic maxillary expression. Images are representative maximum-intensity projections. Scale bars, 20 μm.

(E) Schematized shifts in arch gene expression territories. See Figure S4 for expression patterns of non-mandibular genes.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx2a TU standard conditions Prim-5 pharyngeal arch 1 neural crest cell ISH
Prim-5 pharyngeal arch 2 neural crest cell ISH
Prim-25 pharyngeal arch 1 neural crest cell ISH
Prim-25 pharyngeal arch 2 neural crest cell ISH
el159Tg; el662Tg (TU) standard conditions Prim-5 pharyngeal arch 1 neural crest cell ISH
Prim-5 pharyngeal arch 2 neural crest cell ISH
Prim-25 pharyngeal arch 1 neural crest cell ISH
Prim-25 pharyngeal arch 2 neural crest cell ISH
nr2f1bel523/+; nr2f2el506/el506; nr2f5el611/el611 (TU) standard conditions Prim-25 pharyngeal arch 1 neural crest cell ISH
Prim-25 pharyngeal arch 2 neural crest cell ISH
nr2f1bel523/el523; nr2f2el506/el506; nr2f5el611/el611 (TU) standard conditions Prim-25 pharyngeal arch 1 neural crest cell ISH
Prim-25 pharyngeal arch 2 neural crest cell ISH
nr2f2el506/el506; nr2f5el611/el611 (TU) standard conditions Prim-5 pharyngeal arch 1 neural crest cell ISH
Prim-5 pharyngeal arch 2 neural crest cell ISH
dlx3b TU standard conditions Prim-25 mandibular arch skeleton ISH
el159Tg; el662Tg (TU) standard conditions Prim-25 mandibular arch skeleton ISH
nr2f1bel523/el523; nr2f2el506/el506; nr2f5el611/el611 (TU) standard conditions Prim-25 mandibular arch skeleton ISH
Prim-25 maxilla ISH
dlx5a TU standard conditions Prim-5 mandibular arch skeleton ISH
Prim-25 mandibular arch skeleton ISH
el159Tg; el662Tg (TU) standard conditions Prim-5 mandibular arch skeleton ISH
Prim-25 mandibular arch skeleton ISH
nr2f1bel523/el523; nr2f2el506/el506; nr2f5el611/el611 (TU) standard conditions Prim-25 mandibular arch skeleton ISH
Prim-25 maxilla ISH
nr2f2el506/el506; nr2f5el611/el611 (TU) standard conditions Prim-5 mandibular arch skeleton ISH
Prim-5 maxilla ISH
dlx6a TU standard conditions Prim-5 mandibular arch skeleton ISH
Prim-25 mandibular arch skeleton ISH
el159Tg; el662Tg (TU) standard conditions Prim-5 mandibular arch skeleton ISH
Prim-25 mandibular arch skeleton ISH
nr2f1bel523/el523; nr2f2el506/el506; nr2f5el611/el611 (TU) standard conditions Prim-25 mandibular arch skeleton ISH
Prim-25 maxilla ISH
nr2f2el506/el506; nr2f5el611/el611 (TU) standard conditions Prim-5 mandibular arch skeleton ISH
Prim-5 maxilla ISH
hand2 TU standard conditions Prim-25 pharyngeal arch 1 ISH
el159Tg; el662Tg (TU) standard conditions Prim-25 Not Detected pharyngeal arch 1 ISH
nr2f1bel523/+; nr2f2el506/el506; nr2f5el611/el611 (TU) standard conditions Prim-25 pharyngeal arch 1 ISH
msx1a TU standard conditions Prim-25 mandibular arch skeleton ISH
el159Tg; el662Tg (TU) standard conditions Prim-25 mandibular arch skeleton ISH
nr2f1bel523/+; nr2f2el506/el506; nr2f5el611/el611 (TU) standard conditions Prim-25 mandibular arch skeleton ISH
Prim-25 maxilla ISH
nkx3-2 TU standard conditions Prim-25 pharyngeal arch 1 ISH
el159Tg; el662Tg (TU) standard conditions Prim-25 Not Detected pharyngeal arch 1 ISH
nr2f1bel523/el523; nr2f2el506/el506; nr2f5el611/el611 (TU) standard conditions Prim-25 maxilla ISH
Prim-25 pharyngeal arch 1 ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
el159Tg; el662Tg (TU) standard conditions Prim-5 mandibular arch skeleton dlx5a expression decreased distribution, abnormal
Prim-5 mandibular arch skeleton dlx6a expression decreased distribution, abnormal
Prim-25 mandibular arch skeleton dlx3b expression decreased distribution, abnormal
Prim-25 mandibular arch skeleton dlx5a expression decreased distribution, abnormal
Prim-25 mandibular arch skeleton dlx6a expression decreased distribution, abnormal
Prim-25 mandibular arch skeleton msx1a expression decreased distribution, abnormal
Prim-25 pharyngeal arch 1 hand2 expression absent, abnormal
Prim-25 pharyngeal arch 1 nkx3-2 expression absent, abnormal
nr2f1bel523/+; nr2f2el506/el506; nr2f5el611/el611 (TU) standard conditions Prim-25 mandibular arch skeleton msx1a expression increased distribution, abnormal
Prim-25 maxilla msx1a expression increased amount, abnormal
Prim-25 maxilla msx1a expression increased distribution, abnormal
Prim-25 pharyngeal arch 1 hand2 expression increased distribution, abnormal
nr2f1bel523/el523; nr2f2el506/el506; nr2f5el611/el611 (TU) standard conditions Prim-25 mandibular arch skeleton dlx3b expression increased distribution, abnormal
Prim-25 mandibular arch skeleton dlx5a expression increased distribution, abnormal
Prim-25 mandibular arch skeleton dlx6a expression increased distribution, abnormal
Prim-25 maxilla dlx3b expression increased amount, abnormal
Prim-25 maxilla dlx3b expression increased distribution, abnormal
Prim-25 maxilla dlx5a expression increased amount, abnormal
Prim-25 maxilla dlx5a expression increased distribution, abnormal
Prim-25 maxilla dlx6a expression increased amount, abnormal
Prim-25 maxilla dlx6a expression increased distribution, abnormal
Prim-25 maxilla nkx3-2 expression increased amount, abnormal
Prim-25 pharyngeal arch 1 nkx3-2 expression increased distribution, abnormal
nr2f2el506/el506; nr2f5el611/el611 (TU) standard conditions Prim-5 mandibular arch skeleton dlx5a expression increased distribution, abnormal
Prim-5 mandibular arch skeleton dlx6a expression increased distribution, abnormal
Prim-5 maxilla dlx5a expression increased amount, abnormal
Prim-5 maxilla dlx5a expression increased distribution, abnormal
Prim-5 maxilla dlx6a expression increased amount, abnormal
Prim-5 maxilla dlx6a expression increased distribution, abnormal
Acknowledgments:
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Reprinted from Developmental Cell, 44(3), Barske, L., Rataud, P., Behizad, K., Del Rio, L., Cox, S.G., Crump, J.G., Essential Role of Nr2f Nuclear Receptors in Patterning the Vertebrate Upper Jaw, 337-347.e5, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell