FIGURE

Fig. 4

ID

ZDB-FIG-180511-37

Publication

Berger et al., 2018 - In Vivo Function of the Chaperonin TRiC in α-Actin Folding during Sarcomere Assembly

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Fig. 4

All cct Mutants Show Retina Degeneration, Except for cct5^tf212b

(A) H&E-stained sections of 3-dpf-old cct3^sa1761 mutants showed severe retina degeneration (n = 11 per genotype) accompanied by abundant apoptosis detected by TUNEL assay (n = 6 per genotype).

(B) At 3 dpf, retina degeneration was also apparent on H&E-stained sections of the insertion mutant cct5^hi2972Tg but not of the missense mutant cct5^tf212b (n = 11 per genotype).

(C) Pursuant to the retina degeneration, immunohistochemistry with antibodies against acetylated α-tubulin detected neurite defects in the tectum (arrowheads) of cct3^sa1761 and cct5^hi2972Tg but not cct5^tf212b homozygotes (n = 4 per genotype).

See also Figure S6.

Expression Data

Antibody:	Ab1-tuba
Fish:	WT cct3^{sa1761/sa1761} cct5^{hi2972bTg/hi2972bTg} cct5^{tf212b/tf212b}
Anatomical Term:	optic tectum neuron projection
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cct3^{sa1761/sa1761} cct5^{hi2972bTg/hi2972bTg} cct5^{tf212b/tf212b}
Observed In:	optic tectum optic tectum neuron projection optic tectum neuron projection development retina retina apoptotic process
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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