FIGURE

Fig. 3-S1

ID

ZDB-FIG-180417-23

Publication

Rogers et al., 2017 - Nodal patterning without Lefty inhibitory feedback is functional but fragile

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Fig. 3-S1

Expression of nodal, lefty, and Nodal target genes in lefty1^-/-;lefty2^-/- mutants during gastrulation.

Wild type embryos, lft1^-/- embryos, lft2^-/- embryos, and (to generate lft1^-/-;lft2^-/- embryos) progeny from lft1^+/-;lft2^-/- incrosses, lft1^-/-;lft2^-/- incrosses, or lft1^-/-;lft2^-/- X lft1^+/-;lft2^-/- crosses were fixed at the indicated stages. In situ hybridization was performed using probes against the following Nodal target genes: lft1 (A), lft2 (B), sqt (C), cyc (D), tb/no tail (E), and sox17 (F). Dorsal views. After imaging, embryos from lft1^+/-;lft2^-/- incrosses or lft1^-/-;lft2^-/- X lft1^+/-;lft2^-/- crosses were genotyped (see Materials and methods for details). Upregulation of Nodal target genes was most dramatic in lft1^-/-;lft2^-/- mutants, though lft1^-/- mutants tended to exhibit slight upregulation as well. Note that expression of tb/no tail at 30% epiboly was normal in lft1^-/-;lft2^-/- mutants, in contrast to the dramatic upregulation at 30% epiboly observed in double lefty morphants (Agathon et al., 2001).

Expression Data

Genes:	lft1 lft2 ndr1 ndr2 sox17 tbxta
Fish:	WT lft1^a145/a145 lft2^a146/a146 lft2^a146/a146; lft1^a145/a145
Anatomical Terms:	axis blastodisc blastodisc anatomical margin endoderm margin
Stage Range:	30%-epiboly to 75%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lft1^a145/a145 lft2^a146/a146; lft1^a145/a145
Observed In:	axis blastodisc anatomical margin endoderm margin
Stage Range:	Sphere to 75%-epiboly

Phenotype Detail

Acknowledgments

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