Fig. 10

Application of the presented framework to 3D+t light-sheet microscopy images of zebrafish embryos

The panels show the maximum intensity projections on the XY, YZ and XZ planes. Seed points were extracted using the LoGNSM+F+U method and the improved seed points were used for segmentation using the TWANG+U method. Extracted objects of two opposite views were combined using a segment-based fusion approach (S4 Note) and the fused object locations were then used for tracking (TWANG+U+NN). The first three columns show results of a single view at approximately 8 hours post fertilization (hpf) with the color-code according to the FSMD values of the extracted objects (red over blue to green for low, medium and high FSMD values). The last column shows the results after multiview fusion and tracking at about 10 hpf using a cell density for coloring. In addition, the embryo was oriented along the coordinate axes, such that the anteroposterior axis was aligned with the Y axis (animal pole on the top) and the dorsoventral axis aligned with the X axis (dorsal to the right) [37]. Data were taken from our previously published work [37–39, 47]. Scale bar, 100 μm.