FIGURE

Fig. 3

ID

ZDB-FIG-180306-11

Publication

Stones et al., 2017 - Zebrafish (Danio rerio) as a Vertebrate Model Host To Study Colonization, Pathogenesis, and Transmission of Foodborne Escherichia coli O157.

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Fig. 3

EHEC triggers transient neutrophil recruitment to the intestinal region and surrounding tissue upon colonization. mpo::gfp zebrafish larvae were exposed to 10⁸ CFU/ml foodborne EHEC::mCherry for 2 h, washed, mounted live in low-melting-temperature agarose-containing tricaine, and imaged for 12 h at 32°C. (A) Representative frames showing intestinal tract of infected fish after 0 h (Ai), 4 h (Aii), 8 h (Aiii), and 12 h (Aiv). Accumulation of fecal matter at the anal opening is also visible. (B) Neutrophils in the intestinal region and surrounding tissue (counts from the intestinal region as shown in panel A) were enumerated. Individual data points from infected fish (gray) and uninfected fish (blue), means (n = 5 per condition), and SD are shown. The statistical significance of results of comparisons between infected and uninfected fish for individual time points was determined using Student’s t test (*, P < 0.05; **, P < 0.01). ns, not significant.

Expression Data

Gene:	GFP
Fish:	i114Tg
Condition:	bacterial treatment by diet: Escherichia coli O157:H7
Anatomical Term:	neutrophil
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	i114Tg
Condition:	bacterial treatment by diet: Escherichia coli O157:H7
Observed In:	intestine anatomical region neutrophil neutrophil chemotaxis
Stage:	Day 4

Phenotype Detail

Acknowledgments

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