FIGURE

Fig. 5

ID

ZDB-FIG-180222-22

Publication

Akerberg et al., 2017 - Histone demethylases Kdm6ba and Kdm6bb redundantly promote cardiomyocyte proliferation during zebrafish heart ventricle maturation

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Fig. 5

Kdm6b proteins enable coordinated endocardial and myocardial proliferation associated with early stage ventricle maturation. (A-C) Confocal immunofluorescence images of sagittal heart sections from 5 dpf kdm6b- and kdm6a/kdm6b-deficient Tg(kdrl:EGFP) larvae stained with anti-GFP (green, endocardium), anti-myosin heavy chain (MF20, red, myocardium), and anti-S46 (magenta, atrial muscle) antibodies. (B, D) Cardiac troponin (CT3) antibody staining of superficial sections through the heart of 5 dpf kdm6bb^-/- and kdm6ba^-/-; kdm6bb^-/- larvae. B’ and D’ are zoomed images of the dashed yellow box regions. (E-F) Confocal imaged sagittal sections of 5 dpf control and combined kdm6b-deficient Tg(kdrl:EGFP) embryos stained with wheat germ agglutinin (WGA, grey) as well as anti-GFP (green in overlay panels, endocardium) and anti-PCNA (magenta in overlay panels) antibodies. Yellow boxes in E and F indicate zoomed regions shown in E’-F’’’. Arrows and arrowheads mark PCNA-positive endocardial and myocardial cells, respectively. (G) Scatterplot graphs showing the number of PCNA positive endocardial and myocardial cells within the outer curvature ventricular wall scored from matched sagittal heart sections of 5 dpf control and kdm6ba^-/-; kdm6bb^-/- larvae. Each point represents a distinct fish. P-values are from two-tailed Student's t-tests. (H, I) Confocal heart images of sagittal sectioned 5 dpf control and kdm6a/kdm6b-deficient larvae stained for EdU incorporation (cyan, proliferating cells) and with anti-myosin heavy chain antibody (red, MF20, cardiomyocytes).

Expression Data

Genes:	EGFP pcna
Antibodies:	Ab-MF20 Ab-S46 Ab3-tnnt
Fish:	kdm6ba^b1308/+; kdm6bb^b1307/b1307; s843Tg kdm6ba^b1308/b1308; kdm6bb^b1307/b1307; s843Tg
Anatomical Terms:	atrial myocardium endocardium heart myocardium
Stage Range:	Day 5 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	kdm6ba^b1308/+; kdm6bb^b1307/b1307; s843Tg kdm6ba^b1308/b1308; kdm6bb^b1307/b1307; s843Tg
Observed In:	endocardium endocardium cell population proliferation myocardium myocardium cell population proliferation
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 426(1), Akerberg, A.A., Henner, A., Stewart, S., Stankunas, K., Histone demethylases Kdm6ba and Kdm6bb redundantly promote cardiomyocyte proliferation during zebrafish heart ventricle maturation, 84-96, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.