ZFIN ID: ZDB-FIG-180208-4
Donat et al., 2018 - Heg1 and Ccm1/2 proteins control endocardial mechanosensitivity during zebrafish valvulogenesis. eLIFE   7:e28939 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: Prim-15 to Long-pec

Fig. 1

Mechanosensitive expression of heg1 mRNA and stabilization of Krit1 protein levels by heg1.

(A) RT-qPCR quantifications of heg1 and krit1 mRNA levels at 54 hpf in morphant embryos lacking blood-flow [troponin T type 2a (tnnt2a)], or Krüppel like factors 2a and 2b (klf2a/b) compared with wild-type expression levels. (B) RT-qPCR quantification showing that heat-shock-induced overexpression of klf2a causes significant upregulation of heg1 but not of krit1 expression at 54 hpf. (C–E) Morpholino-mediated knockdown of Heg1 significantly reduces endocardial EGFP-Krit1 protein levels of 30 hpf Tg(fli:GAL4FF)ubs3; Tg(UAS:EGFP-Krit1)pbb21 embryos. Scale bars are 50 µm. Mean values ± SEM of three (tnnt2a MO, hs:klf2a, heg1 MO) or four (klf2a/b MO) individual experiments are shown. Ratio paired (A, B) or unpaired (E) t-test was used to compare each condition with controls (ns: not significant, *p<0.05, ***p<0.001).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP pbb21Tg; ubs3Tg standard conditions Prim-15 endocardium IFL
pbb21Tg; ubs3Tg + MO1-heg1 standard conditions Prim-15 endocardium IFL
heg1 WT + MO1-tnnt2a standard conditions Long-pec whole organism RTPCR
WT + MO2-klf2a + MO2-klf2b standard conditions Long-pec whole organism RTPCR
pbb22Tg heat shock Long-pec whole organism RTPCR
krit1 WT + MO1-tnnt2a standard conditions Long-pec whole organism RTPCR
WT + MO2-klf2a + MO2-klf2b standard conditions Long-pec whole organism RTPCR
pbb22Tg heat shock Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tnnt2a standard conditions Long-pec blood circulation arrested, abnormal
Long-pec whole organism heg1 expression decreased amount, abnormal
WT + MO2-klf2a + MO2-klf2b standard conditions Long-pec blood circulation arrested, abnormal
Long-pec whole organism heg1 expression decreased amount, abnormal
pbb21Tg; ubs3Tg + MO1-heg1 standard conditions Prim-15 endocardium EGFP expression decreased amount, abnormal
pbb22Tg heat shock Long-pec whole organism heg1 expression increased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife