ZFIN ID: ZDB-FIG-180118-42
Zabinyakov et al., 2017 - Characterization of the first knock-out aldh7a1 zebrafish model for pyridoxine-dependent epilepsy using CRISPR-Cas9 technology. PLoS One   12:e0186645 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Days 7-13

Fig. 4 EEG results of knock-out homozygous aldh7a1 embryos and wild type embryos at 9 dpf. A) This shows spike discharges in a homozygous with no response to 100 μM diazepam, but almost normalization of EEG on 25 mM pyridoxine compared to wild type. B) This shows another homozygous with no response to phenobarbital, but almost normalization of EEG on pyridoxine (25 mM) compared to wild type. C) This shows normal EEG with spikes on pentylenetetrazole, and normalization of EEG on diazepam.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: pentetrazol Days 7-13 regulation of neuronal action potential disrupted, abnormal
WT chemical treatment: diazepam, chemical treatment: pentetrazol Days 7-13 regulation of neuronal action potential disrupted, ameliorated
aldh7a1zf2074/zf2074 control Days 7-13 regulation of neuronal action potential disrupted, abnormal
aldh7a1zf2074/zf2074 chemical treatment: diazepam Days 7-13 regulation of neuronal action potential disrupted, abnormal
aldh7a1zf2074/zf2074 chemical treatment: phenobarbital Days 7-13 regulation of neuronal action potential disrupted, abnormal
aldh7a1zf2074/zf2074 chemical treatment: pyridoxine Days 7-13 regulation of neuronal action potential disrupted, ameliorated
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One