FIGURE

Fig. 2

ID

ZDB-FIG-171121-7

Publication

Shi et al., 2016 - Combretastatin A-4 efficiently inhibits angiogenesis and induces neuronal apoptosis in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Effects of CA-4 treatment on vascular and central nervous systems in the trunk of Tg(kdrl:EGFP::huc:mcherry) and Tg(fli1a:nEGFP) zebrafish embryos at 55 hpf.

(A–D) ISV phenotypes of control group and 7, 8, 9 ng/ml CA-4 treated groups. Scale bar, 100 μm. (A′–D″) Zoomed in images of regions in dash line rectangles of panel (A–D). Scale bar, 50 μm. (E–H) mCherry positive cells in neural tubes of control group and 7, 8, 9 ng/ml CA-4 treated groups. Scale bar, 100 μm. (E′–H′) Zoomed in images of regions in dash line rectangles of panel E-H. Scale bar, 50 μm. (I–L) Merged images of (A–H). Scale bar, 100 μm. (I′–L′) Zoomed in images of regions in dash line rectangles of panel I-L. Scale bar, 50 μm. (M–P) Diagrams of ISVs and neuronal precursor cells in different groups. Scale bar, 50 μm. (Q–T) Endothelial nuclei of control group and 7, 8, 9 ng/ml CA-4 treated groups. Scale bar, 100 μm. (U–X) Statistical analyses of ISV length, ISV absence ratio, number of neuronal precursor cell in each somite, and mean endothelial cell number of each ISV in control group and 7, 8, 9 ng/ml CA-4 treated groups. Data were expressed as the mean ± S.E.M. (n = 8). **P < 0.01 and ***P < 0.001 vs. control group.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.