FIGURE

Fig. 1

ID

ZDB-FIG-171113-35

Publication

de Boeck et al., 2016 - Smad6 determines BMP-regulated invasive behaviour of breast cancer cells in a zebrafish xenograft model

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Fig. 1

Overexpression of Smad6 in MCF10A M2 blocks BMP signalling and potentiates invasion.

(a,b) Western blot analysis of total protein from control (CO) and Flag-Smad6 stable expression (S6) MCF10A M2 cell lines treated with 50 ng/ml BMP6 or 0.5 ng/ml TGF-β for 1 hr (a, *aspecific bands) and 50 ng/ml BMP6 or 5 ng/ml TGF-β for 24 hrs (b). (c) Gelatin zymogram showing MMP9 activity in conditioned medium of mock and TGF-β treated MCF10A M2 cells. (d,e) Representative images of 6 dpi zebrafish larvae showing the invasion of control (CO) (d) and Smad6 overexpression (S6) (e) MCF10A M2 cells. (f) Quantification of invasive cluster numbers in CO and S6 MCF10A M2 injected zebrafish larvae. R.I.: Quantification of relative intensity. * 0.01 < p < 0.05. Scale bar: 250 μm.

Expression Data

Gene:	EGFP
Fish:	y1Tg
Condition:	cancer xenotransplantation
Anatomical Term:	vasculature
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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