Fig. 3

Direct hair cell counts confirm otoprotection. Ber, E6, Her and Iso robustly protect hair cells from 200 μM neomycin after acute exposure. (A–F) Representative low magnification (A–C) and high magnification (D–F) images of myo6b: EGFP transgenic fish treated with the indicated conditions. White circles in (A–C) denote the neuromasts shown in (D–F). Scale bar in (A) = 40 μm and applies to panels (A–C), scale bar in (D) = 10 μm and applies to panels (D–F). (G–I) Representative images of brn3c: mGFP hair cells treated with the indicated conditions. (G) The dotted line encircles a hair cell. Scale bar in (G) = 10 μm and applies to panels (G–I). (J) Direct counts of hair cells in five neuromasts per fish demonstrate significant hair cell protection in *AB fish labeled with anti-parvalbumin (black bars, one-way ANOVA, F _(4,60) = 46.58, p < 0.0001), Brn3c fish (light gray bars, one-way ANOVA, F_(4,38) = 75.58, p < 0.0001), and myo6: EGFP fish (dark gray bars, one-way ANOVA, F_(4,53) = 14.86, p < 0.0001). N = 8–24 and error bars are ±SEM. and ****p < 0.0001.