FIGURE

Fig. 6

ID
ZDB-FIG-171004-13
Publication
Demy et al., 2017 - Trim33 / Tif1-γ is essential for macrophage and neutrophil mobilisation to developmental or inflammatory cues
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Fig. 6

Bacterial infection is insufficient to recruit mutant myeloid cells. (A–H) In vivo time-lapse imaging of macrophages (A,B,E,F) and neutrophils (C,D,G,H) of monNQ039Tg(mpeg1:mCherry/mpx:gfp) sibling (Sib) (A–D) and mutants (Mut) (E–H) by 60 hpf, 20 min after E. coli injection in the inner ear (A,C,E,G) or 7 h later (B,D,F,H). Bacteria injection in the ear induces a strong recruitment of both macrophages and neutrophils in the siblings (B,D), but almost none in the moonshine mutant (F,H). e, ear; l, lens; r, retina; m, midbrain; h, hindbrain; y, yolk sac. See also Movie 7. (I,J) Quantification of neutrophil mean distance to the ear following injection of GFP-positive B. subtilis in the inner ear by 72 hpf (I) shows a drop during the first 2 hours post injection in the siblings (light curve) and not in the mutant (black curve); accordingly, the number of neutrophils inside the ear of infected larvae constantly increases over time in the siblings (light curve) but not in the mutant (black curve). See also Movie 8.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Conditions:
Observed In:
Stage: Protruding-mouth

Phenotype Detail
Acknowledgments
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