FIGURE

Fig. 7

ID

ZDB-FIG-170901-8

Publication

Gau et al., 2017 - An ancient neurotrophin receptor code; a single Runx/Cbfβ complex determines somatosensory neuron fate specification in zebrafish

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Fig. 7

Loss of runx or cbfb expression affects ion channel expression in the TG.

A-I, Colormetric in situ hybridization for trpv1 (A-C), trpa1b (D-F), and piezo2b (G-I) in the runx3^w144/w144 and cbfb^w128/w128 mutants focusing on the TG at 24hpf and 3dpf. J-L, Antibody staining of GFP (green) in runx3^w144/w144; trpa1b:GFP and cbfb^w128/w128; trpa1b:GFP mutants. M, Quantification of GFP+ cells/ganglion in the TG at 24hpf and 3dpf. Dashed line outlines the eye; Arrowhead, TG. Scale bar: A-I 100 μm, J-L 20μm. Embryos per condition (n = 3–7). ***p<0.001. All error bars represent S.E.M.

Expression Data

Genes:	EGFP piezo2b trpa1b trpv1
Fish:	WT cbfb^w128/w128 (AB) runx3^w144/w144 (AB) a129Tg cbfb^w128/w128; a129Tg (AB) runx3^w144/w144; a129Tg (AB) (all 6)
Anatomical Term:	trigeminal ganglion
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cbfb^w128/w128 (AB) runx3^w144/w144 (AB) cbfb^w128/w128; a129Tg (AB) runx3^w144/w144; a129Tg (AB)
Observed In:	trigeminal ganglion
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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