ZFIN ID: ZDB-FIG-170807-12
Tao et al., 2017 - The pre-rRNA processing factor DEF is rate limiting for the pathogenesis of MYCN-driven neuroblastoma. Oncogene   36(27):3852-3867 Full text @ Oncogene
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 7-13 to Adult

Fig. 4

DEF accelerates tumor onset and increases the penetrance of MYCN-induced neuroblastoma. (a, b) Cumulative frequency of neuroblastoma in two independent DEF stable transgenic lines by Kaplan–Meier analysis. The difference between tumor onset by 29 wpf for TgDEF#1;MYCN (a) and TgDEF#2;MYCN (b) vs TgmCherry;MYCN transgenic fish is significant at P<0.0001 and P<0.0001 (by the log-rank test), respectively. (c, d) A 15 wpf TgDEF transgenic line without detectable EGFP and mCherry-positive tumor in the IRG. (g, h) A 35 wpf TgMYCN transgenic line with an EGFP-positive tumor (arrow) arising in the IRG. (k, l) A 15 wpf TgDEF;MYCN compound transgenic line with an EGFP and mCherry-positive tumor (arrows) arising in the IRG. Scale bars represent 1 mm for c, d, g, h, k and l. (e, f, i, j, m and n) H&E-stained sagittal sections of TgDEF transgenic line (e and f), TgMYCN transgenic line with neuroblastoma (i and j) and TgDEF;MYCN transgenic line with neuroblastoma (m and n). Boxes in e, i and m indicate the IRG, and are magnified in f, j and n, respectively. Arrowheads indicate the nucleoli. Scale bars represent 1 mm for e, i and m, and 20μm for f, j and n. (o) Nucleolar area indicated by H&E staining in the tumor sections of TgMYCN (j) and TgDEF;MYCN (n) transgenic fish was calculated. Horizontal bars indicate the means±s.e.m., which were compared with the two-tailed unpaired Welch t-test, P<0.0001. (p–s) Coimmunostaining of EGFP, mCherry and Fib of the sagittal sections through IRG of TgEGFP (p), TgDEF (q), TgMYCN (r) and TgDEF;MYCN (s) transgenic lines at 6 wpf. Nuclei were stained with DAPI. Scale bar, 4 μm. (t) Nucleolar area indicated by staining with the nucleolar marker Fib in the sections of (p–s) and Figure 2h was calculated. Horizontal bars indicate the means±s.e.m., which were compared with the two-tailed unpaired Welch t-test. E, eye; G, gill; H, heart; I, intestine; IRG, interrenal gland; L, liver; T, testis; wpf, weeks post fertilization.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zdf16Tg (AB) standard conditions Days 30-44 interrenal gland nucleolus increased area, abnormal
Adult interrenal gland neuroblastoma present, abnormal
Adult interrenal gland nucleolus increased area, abnormal
zdf16Tg; zdf31Tg (AB) standard conditions Days 30-44 interrenal gland nucleolus increased area, abnormal
Days 30-44 - Adult interrenal gland neuroblastoma increased amount, exacerbated
zdf16Tg; zdf32Tg (AB) standard conditions Days 30-44 interrenal gland nucleolus increased area, abnormal
Days 30-44 - Adult interrenal gland neuroblastoma increased amount, exacerbated
Days 30-44 - Adult interrenal gland nucleolus increased area, exacerbated
Adult interrenal gland nucleolus increased area, exacerbated
zdf30Tg (AB) standard conditions Days 30-44 interrenal gland nucleolus increased area, exacerbated
zdf31Tg (AB) standard conditions Days 7-13 - Adult interrenal gland neuroblastoma absent, normal
zdf32Tg (AB) standard conditions Days 7-13 - Adult interrenal gland neuroblastoma absent, normal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Oncogene