FIGURE

Fig. 5

ID

ZDB-FIG-170525-5

Publication

Suli et al., 2014 - A targeted gene expression system using the tryptophan repressor in zebrafish shows no silencing in subsequent generations.

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Fig. 5

Gal80 can inhibit nlsTrpR-G4AD. Adults from F2 generations of Tg(CC,myo6b:nlsTrpR-G4AD)^w83 and Tg(CV,tUAS:nlsEos)^w81 were crossed, and a subset of embryos was injected with pTol2-CG2,myo6:Gal80IREStRFP. (A-A′) Hair cell nuclei, as visualized by nlsEos, of one neuromast in an uninjected larva. Note that, because the larvae were not reared in the dark, a portion of nlsEos, which is usually detected in the green channel, was photoconverted and nlsEos was detected in both the green channel (A′) and in the red channel (A). (B-B′) A neuromast in a larva injected with pTol2-CG2,myo6:Gal80IREStRFP. Cells with cytoplasmic tRFP (white arrows, B,B′), which express Gal80, have no nlsEos expression (blue arrows, B′), showing that Gal80 can inhibit Gal4 in the TrpR/tUAS system.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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