FIGURE

Fig. 2

ID

ZDB-FIG-170525-2

Publication

Suli et al., 2014 - A targeted gene expression system using the tryptophan repressor in zebrafish shows no silencing in subsequent generations.

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Fig. 2

Transgenic zebrafish lines. (A,B) Two and 3 day post-fertilization (dpf) embryos from reporter lines visualized by injection of nlsTrpR-G4AD mRNA into one-cell stage embryos. (C) Expression pattern of the myo6b promoter visualized by GFP in a transgenic line. (D-F) Driver lines crossed to reporter lines. Heads of 5 dpf double-transgenic zebrafish larvae showing expression of reporter genes in hair cells. The myo6b promoter drives expression in vestibular (blue arrows) and auditory hair cells and lateral line hair cells (white arrows) (D,E), whereas the ribA promoter drives expression in vestibular (blue arrows) and auditory hair cells, lateral line hair cells (white arrows), bipolar and photoreceptor cells (obscured by retinal pigment cells) and pineal cells (not shown) (F). The lens expression is also visible in D-F (white arrowheads).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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