ZFIN ID: ZDB-FIG-170509-21
Chitramuthu et al., 2017 - Neurotrophic effects of progranulin in vivo in reversing motor neuron defects caused by over or under expression of TDP-43 or FUS. PLoS One   12:e0174784 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 4

PGRN rescues motor axon defect produced by TDP-43 and FUS knockdown or expression of their mutant mRNAs.

Lateral views (anterior to the left; dorsal to the top) of embryos obtained from the HB9:GFP transgenic fish. Fig 4A. Compared to controls (A1) embryos injected with TDP-43 AMO produced truncated axons (A2). Embryos co-injected with hPGRN mRNA (TDP-43 AMO+hPGRN) partially reversed the truncation phenotype (A3). Fig 4B. Compared to control (B1), embryos injected with FUS AMO produced truncated axons (B2). Embryos co-injected with hPGRN mRNA (FUS AMO +hPGRN) reversed the truncation phenotype (B3). Fig 4C. Compared to controls (C1), embryos injected with TDP43 (G348C) produced truncated axons (C2). Embryos co-injected with hPGRN mRNA (TDP43 (G348C)+hPGRN) reversed the truncation phenotype (C3). Fig 4D. Compared to controls (D1), embryos injected with FUS (R521H) produced truncated axons (D2). Embryos co-injected with hPGRN mRNA (FUS (R521H)+hPGRN) reversed the truncation phenotype (D3). Dashed line indicates the horizontal myoseptum. Arrow points to a single axon. Images were captured at 5X magnification and the hatched box was further subject to 4-5X Zoom.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ml2Tg + MO1-fus standard conditions Long-pec axon extension process quality, abnormal
Long-pec CaP motoneuron axon decreased length, abnormal
ml2Tg + MO1-tardbpb standard conditions Long-pec axon extension process quality, abnormal
Long-pec CaP motoneuron axon decreased length, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One