FIGURE
Fig. S6
- ID
- ZDB-FIG-170407-5
- Publication
- Zelina et al., 2014 - Signaling switch of the axon guidance receptor Robo3 during vertebrate evolution
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Fig. S6
Selective rescue of Robo3-/- pontine neurons by mammalian Robo3. (A-D) E17.5 Robo3-/- hindbrain co-electroporated at E13.5 with mouse Robo3A.1 and GFP. Immunostaining for Robo3 (B) and for Barhl1/Robo3 (C). Electroporated pontine neurons send their axons (arrowheads) across the floor plate (dotted line in A-C). (C) The distance separating the floor plate from the front of migrating Barhl1 positive pontine neurons is reduced on the electroporated (rescued) side compared to the opposite (non-electroporated) side (double arrows). See text for quantification. (D) Expression levels of mRobo3A.1-V5 verified by immunoprecipitation from electroporated hindbrain protein extracts. (E-I) E17.5 Robo3-/- hindbrain co-electroporated at E13.5 with zebrafish Robo3A.1 and GFP. Immunostaining for Robo3 (F) and for Barhl1/GFP (G). Note that GFP/Robo3 positive neurons stay in the aberrant dorsal stream and do not approach the midline. (H) is a Robo3-/- embryo electroporated with zRobo3A.1 and processed as whole-mount for in situ hybridization with a zRobo3 probe. The arrows show zRobo3+ pontine neurons in the aberrant stream. (I) Expression levels of zRobo3A.1-myc (arrowhead) verified by immunoprecipitation from electroporated hindbrain protein extracts. (J, K) the mouse Robo3 lacking the CC2-CC3 domain fails to rescue ventral migration. (L, M) pontine neurons and axons from Wnt1:cre;Robo3lox/lox knockout embryo expressing Robo3Y1019F phosphorylation mutant, failed to reach the midline. Scale bars: 60μm in (M), 100 μm in (A-C), 120 μm in (E and L) and (F), 150 μm in (G), 220 μm in (J, K), 400 μm in (H) |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
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