FIGURE

Fig. 6

ID

ZDB-FIG-170314-17

Publication

Meda et al., 2016 - Nerves Control Redox Levels in Mature Tissues Through Schwann Cells and Hedgehog Signaling

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Early HH signaling is necessary for ROS production. (A–C) HH-i added to the water bath from 0 to 24 hpa (blue) or from 8 to 24 hpa (orange) inhibits regeneration, which can be rescued by Smo-A (green). (D–H) Hedgehog inhibition with HH-i (cyclopamine) (D) reduced ROS levels at the amputation plane at 16 hpa (E, F) and stump proliferation at 24 hpa (G, H). (G, H) Immunofluorescence staining for the axonal marker, acetylated tubulin (green), and for the mitotic cell marker, phosphorylated histone H3 (red). (I) Nox-i added to the water bath from 0 to 24 hpa reduced the number of Shh-positive cells detected at 48 hpa. Shh-positive cells were visualized by immunodetection of the GFP in shh:GFP transgenic fish. Error bars represent the SEM (*p < 0.05; ***p < 0.001). n Values are indicated at the bottom of each column of the graphs. HH-i, hedgehog inhibitor, cyclopamine; Smo-A, smoothened agonist.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Antioxid. Redox Signal.