FIGURE

Fig. S9

ID

ZDB-FIG-170208-58

Publication

Davey et al., 2016 - PCP Signaling between Migrating Neurons and their Planar-Polarized Neuroepithelial Environment Controls Filopodial Dynamics and Directional Migration

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Fig. S9

The effect of PCP on protrusion dynamics is dependent on the migratory environment.

(A) Method used to isolate and identify FBMNs in primary culture. Embryos used were Tg(isl1:mTFP);Tg(hoxb1a:RFP) allowing for the differentiation between FBMNs and other branchiomotor neurons labeled by Tg(isl1:mTFP). (B,C) Cultured Tg(isl1:mTFP); Tg(hoxb1a:RFP) FBMNs from a wild type (B) and a vangl2 mutant embryo (C). (B',C') Time-lapse spinning-disc confocal series of boxed region from B and C. (D) Quantitation of filopodial lifetime for cultured FBMNs. Each timelapse was 600 seconds total. p = 0.9044, n.s. (E) Quantitation of the maximum filopodial length for cultured FBMNs. p = 0.0856, n.s. Wild type: N = 8 neurons, 64 filopodia. vangl2^-/-: N = 8 neurons, 61 filodpodia. Graphs represent data as mean ± SEM. Each data point is the average lifetime (D) or maximum length (E) for all the filopodia of one FBMN. Significance was determined using an unpaired, two-tail t-test with Welch’s correction.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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