FIGURE

Fig. S5

ID

ZDB-FIG-170208-21

Publication

Bielczyk-Maczyńska et al., 2015 - The Ribosome Biogenesis Protein Nol9 Is Essential for Definitive Hematopoiesis and Pancreas Morphogenesis in Zebrafish

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Fig. S5

Gene knockdown studies using nol9 morpholino confirm the pancreas phenotype observed in nol9^{sa1022/sa1022} mutants.

(A) General morphology of 96 hpf larvae injected with either standard control morpholino (std MO) or a morpholino targeting the translational start site of the nol9 transcript (nol9 ATG MO). Numbers represent larvae with the displayed phenotype out of the total number of larvae examined. (B) Representative confocal images of the pancreas of 96 hpf Tg(ptf1a:EGFP;ins:mCherry) larvae injected with std MO or nol9 ATG MO. While the ptf1a⁺ exocrine pancreas appeared smaller in larvae injected with nol9 ATG MO compared to std MO, the ins⁺ endocrine pancreas was comparable between the groups. (C) The average volume of the ptf1a⁺ exocrine pancreas in 96 hpf larvae injected with std MO (n = 19) or nol9 ATG MO (n = 25). Data are represented as the mean +/- SEM, Student’s t-test, **, p<0.01.

Expression Data

Genes:	EGFP mCherry
Fish:	jh1Tg; jh2Tg jh1Tg; jh2Tg + MO1-nol9
Knockdown Reagent:	MO1-nol9
Anatomical Terms:	endocrine pancreas exocrine pancreas
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	jh1Tg; jh2Tg + MO1-nol9
Knockdown Reagent:	MO1-nol9
Observed In:	endocrine pancreas exocrine pancreas
Stage:	Day 5

Phenotype Detail

Acknowledgments

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