FIGURE

Fig. 3

ID

ZDB-FIG-170208-11

Publication

Bielczyk-Maczyńska et al., 2015 - The Ribosome Biogenesis Protein Nol9 Is Essential for Definitive Hematopoiesis and Pancreas Morphogenesis in Zebrafish

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Fig. 3

nol9 mutation affects the development of the exocrine but not the endocrine pancreas.

(A) Representative single channel confocal images of the pancreas of 96 hpf Tg(ptf1a:EGFP;ins:mCherry) larvae. nol9^{sa1022/sa1022} larvae were characterized by smaller ptf1a-positive area (green) and similar ins-positive area (red) compared to nol9^+/+ siblings. (B) TEM pictures of the exocrine pancreas of nol9^+/+ and nol9^{sa1022/sa1022} larvae at 120 hpf. The white arrow denotes endoplasmic reticulum in the nol9^+/+ cell. Asterisks indicate zymogen granules. Arrowheads indicate mitochondria. Scale bar: 2 μm. (C-D) Confocal images of the pancreas of Tg(ptf1a:EGFP) larvae subjected to immunohistochemistry against α-Carboxypeptidase-a (α-Cpa) (C) and α-Cytokeratin (D) at 120 hpf. (C) The exocrine pancreas differentiation marker α-Cpa (red) was detected in nol9^+/+ and nol9^{sa1022/sa1022} siblings. (D) The pancreatic ducts expressing α-Cytokeratin (red) are not apparent in nol9^{sa1022/sa1022} mutants, in contrast to the ductal network clearly visible in nol9^+/+ siblings.

Expression Data

Genes:	EGFP mCherry
Antibodies:	Ab2-cpa1 Ab4-krt18
Fish:	jh1Tg jh1Tg; jh2Tg nol9^{sa1022/sa1022}; jh1Tg nol9^{sa1022/sa1022}; jh1Tg; jh2Tg
Anatomical Terms:	endocrine pancreas exocrine pancreas pancreatic B cell pancreatic duct
Stage Range:	Day 4 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nol9^{sa1022/sa1022} nol9^{sa1022/sa1022}; jh1Tg nol9^{sa1022/sa1022}; jh1Tg; jh2Tg
Observed In:	endocrine pancreas exocrine pancreas exocrine pancreas mitochondrion exocrine pancreas rough endoplasmic reticulum exocrine pancreas zymogen granule pancreatic duct
Stage Range:	Day 4 to Day 5

Phenotype Detail

Acknowledgments

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