ZFIN ID: ZDB-FIG-170201-21
Liu et al., 2016 - The Machado-Joseph Disease Deubiquitinase Ataxin-3 Regulates the Stability and Apoptotic Function of p53. PLoS Biology   14:e2000733 Full text @ PLoS Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Prim-5

Fig. S3

Ataxin-3 expression-induced cell death occurs in cells and in HuC positive brain regions in zebrafish.

(A) Flow cytometry analysis using Annexin V-FITC/PI staining in HCT116 p53+/+ cells. (B and C) Dorsal views with anterior to the top of Tg(HuC:EGFP) embryos. Colocalization of HuC:EGFP (green) and TUNEL positive foci (red) in the telencephalon region (B) and diencephalon/hindbrain (C). Tg(HuC:EGFP) transgenic embryos uninjected control (UIC) or injected with ataxin-3 were collected for TUNEL staining at 24 hpf. Scale bars, 20 μm for B and 50 μm for C.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP knu3Tg standard conditions Prim-5 hindbrain neuron IHC
Prim-5 midbrain neuron IHC
Prim-5 telencephalon neuron IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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