Ataxin-3 expression-induced cell death occurs in cells and in HuC positive brain regions in zebrafish.
(A) Flow cytometry analysis using Annexin V-FITC/PI staining in HCT116 p53+/+ cells. (B and C) Dorsal views with anterior to the top of Tg(HuC:EGFP) embryos. Colocalization of HuC:EGFP (green) and TUNEL positive foci (red) in the telencephalon region (B) and diencephalon/hindbrain (C). Tg(HuC:EGFP) transgenic embryos uninjected control (UIC) or injected with ataxin-3 were collected for TUNEL staining at 24 hpf. Scale bars, 20 μm for B and 50 μm for C.