FIGURE

Fig. 7

ID
ZDB-FIG-161222-36
Publication
Alvarado et al., 2016 - Wdr68 Mediates Dorsal and Ventral Patterning Events for Craniofacial Development
Other Figures
All Figure Page
Back to All Figure Page
Fig. 7

Inhibition of TGF-β signaling partially rescues M cartilage and edn1 expression in wdr68hi3812/hi3812 zebrafish.

(A-D) Ventral views of 5dpf Alcian stained craniofacial cartilages of zebrafish raised at 32°C and treated with DMSO or 10μM SB431542 at 14- to 15-somites stage. A) Wildtype zebrafish treated with DMSO control. Red arrow indicates M-PQ joint region. B) wdr68hi3812/hi3812 mutants treated with DMSO control show a lack of M cartilage. C) Wildtype zebrafish treated with 10μM SB431542 show normal craniofacial cartilage formation. D) wdr68hi3812/hi3812 mutants treated with 10μM SB431542 show a partial rescue of M. E) SB431542-treated mutants show a significantly reduced fraction of severe defects compared to the control group (p<0.012). (F-I) Dorsal views of edn1 ISH analysis on 22-somites stage embryos treated with DMSO or 10μM SB431542 starting at the 14- to 15-somites stage. F) Wildtype embryos treated with DMSO control. G) wdr68hi3812/hi3812 mutants treated with DMSO control show lack of edn1 expression. H) Wildtype embryos treated with SB431542 show similar expression compared to wild type. I) wdr68hi3812/hi3812 mutants treated with SB431542 show partial restoration of edn1 expression.

Expression Data
Gene:
Fish:
Conditions:
Anatomical Term:
Stage: 26+ somites

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Conditions:
Observed In:
Stage Range: 26+ somites to Day 5

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One