FIGURE

Fig. 2

ID

ZDB-FIG-160926-34

Publication

Bhuiyan et al., 2016 - Acinetobacter baumannii phenylacetic acid metabolism influences infection outcome through a direct effect on neutrophil chemotaxis

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Fig. 2

Neutrophils are indispensible for zebrafish survival from A. baumannii infection. (A) Phagocytosed A. baumannii (pHrodo-dextran stain, white arrow) is handled within acidic phagolysosomes within a neutrophilic vacuole at 50-min postinfection [Tg(mpx:GFP)^il14 embryos with green fluorescent neutrophils were used]. (Scale bar: 20 µm.) Survival of neutrophil-depleted embryos (csf3r MO) (B) or macrophage-depleted embryos (irf8 MO) (C) after bloodstream infection with A. baumannii. Comparison is made vs. A. baumannii-infected embryos injected with control morpholino (Ctrl MO; P < 0.001; n = 30 embryos, three biological replicates).

Expression Data

Gene:	GFP
Fish:	i114Tg
Condition:	bacterial treatment by injection: Acinetobacter baumannii ATCC 17978
Anatomical Term:	neutrophil
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA