FIGURE

Fig. S6

ID

ZDB-FIG-160825-2

Publication

Mahmoud et al., 2016 - TWIST1 Integrates Endothelial Responses to Flow in Vascular Dysfunction and Atherosclerosis

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Fig. S6

twist1 regulated sub-intestinal vein sprouting in embryos. (A) In situ hybridisation analysis of twist1a, twist1b or twist2 expression in embryos at 52 or 75 hpf. Scale bar, 100 µm. SIV region is indicated using a broken line. Data shown are representative of the majority of embryos analysed (proportion indicated lower right in each panel). (B) Embryos (Tg(fli1:EGFP;gata1:dsRed)) were treated with twist1b mRNA (to enforce expression) or with mCherry mRNA as a control. They were studied at 75 hpf (flow was established as evidenced by gata1-positive red blood cells). Confocal microscopy was used to visualise angiogenic sprouts (arrows). The number of angiogenic sprouts was quantified for multiple embryos and mean values +/- SEM are shown. Representative images are shown. Scale bar, 500 µm. *** p<0.001 using an unpaired t-test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Circ. Res.