FIGURE

Fig. 9

ID

ZDB-FIG-160824-8

Publication

Fontenille et al., 2014 - Microtubule-associated protein 9 (Map9/Asap) is required for the early steps of zebrafish development

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Fig. 9

In situ hybridization of selected genes of the nodal pathway that are involved in endoderm development. Embryos injected at the 1-cell stage with 1 pmol of control MOmis or MOex5, were fixed at the indicated times that correspond in MOmis-injected control embryos to the following developmental stages: dome (4_1/3 hpf), germ-ring (5_2/3 hpf), and shield (6 hpf). In situ hybridization was performed using antisense oep (nodal co-receptor), sox32 (downstream of oep) and sox17 (activated by sox32 and required for endoderm specification) RNA probes. In map9 morphants, expression of sox32 and sox17 was restricted to a portion of the blastoderm margin at the shield stage (arrowheads, B8, C8).

Expression Data

Genes:	sox17 sox32
Fish:	slc24a5^b1/b1 slc24a5^b1/b1 + MO2-map9
Knockdown Reagent:	MO2-map9
Anatomical Terms:	margin presumptive endoderm yolk syncytial layer
Stage Range:	Dome to Germ-ring

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slc24a5^b1/b1 + MO2-map9
Knockdown Reagent:	MO2-map9
Observed In:	margin
Stage:	Shield

Phenotype Detail

Acknowledgments

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