FIGURE

Fig. 7

ID
ZDB-FIG-160414-16
Publication
Langdon et al., 2016 - Split top: A maternal cathepsin B that regulates dorsoventral patterning and morphogenesis
Other Figures
All Figure Page
Back to All Figure Page
Fig. 7

Cathepsin Ba is deficient in split top mutant embryos. (A) Schematic of the split top interval on chromosome 17. (B) ctsba mRNA expression in wild-type and split top mutant embryos. The genotype and strength of the split top mutant phenotype. (C) Representative phenotypes of uninjected and ctsba-injected wild-type and split top mutant embryos, and (D) bar graphs showing rescue. (E) ctsba mRNA injection in the yolk cell of mid-blastula (high to dome stage) embryos from two homozygous split top mutant females did not rescue the lysis or dorsalized phenotypes. (F) Whole-mount 60% epiboly (early gastrula) embryos stained with actin (red) and DAPI (blue). All uninjected split top mutants exhibited large patches of actin-deficient YCL regions. In the right embryo injected with ctsba mRNA, the gap between the YSN/EVL and the deep cells is rescued, along with partial rescue of the actin cytoskeleton; 3 of 33 embryos showed no actin staining. Scale bar: 140µm. (G) E-64-injected wild-type embryos show expanded chordin expression (animal pole view, dorsal to right). Bar graphs show 1dpf phenotypic distributions. (H) Mis-sense mutation made in the catalytic domain of Ctsba. Bar graphs show 1dpf phenotypic distributions of this mutant mRNA injected into wild-type embryos or embryos from three different split top mutant females.

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage Range: Sphere to Shield

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: Sphere to Prim-5

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development