FIGURE

Fig. 8

ID
ZDB-FIG-160407-12
Publication
Shiu et al., 2016 - Knockdown of zebrafish YY1a can downregulate the phosphatidylserine (PS) receptor expression, leading to induce the abnormal brain and heart development
Other Figures
All Figure Page
Back to All Figure Page
Fig. 8

Injection of PSR mRNA rescued embryos from YY1a morpholino-induced defects. YY1-MO (25 ng) and PSR mRNA (1 ng) were co-injected at the one-to-two cell stage, and embryos were assessed at 18.5 hpf, 48 hpf and 3 dpf. a Apoptotic cell analysis by TUNEL assay. Control-MO and YY1-MO (25 ng per embryo) or YY1-MO (25 ng per embryo) plus PSR mRNA (1 ng) were injected at the one-cell stage to express extra PS receptor. The embryos were fixed and observed at the different stages postfertilization (pf). TUNEL stained embryos (all at 18.5 hpf) are shown in A:a (Control-MO), A:b (YY1-MO group) and A:c (extra PSR mRNA group). The TUNEL-positive cells under the fluorescence microscope are considered apoptotic, especially in A:b (indicated by arrows). Bars indicate 100 µm. (B) Rescued embryos show normal morphology in the brain, heart, and somites. The normal morphology in the brain and heart (indicated by arrows) seen in rescued embryos (panel c, at 24 hpf; f, at 48 hpf; i, at 72 hpf) is in contrast to the deformed brains (indicated by black arrows) and hearts (indicated by red arrows) seen in YY1-MO knockdown embryos (panel b, at 24 hpf; e, at 48 hpf; h, at 72 hpf; indicated by arrows). The control-MO-injected (panel a, at 24 hpf; d, at 48 hpf; g, at 72 hpf) are the negative controls. Bars indicate 200 µm. c The ability of PSR mRNA injection to rescue embryos from morphological deformities during development at 72 hpf was estimated

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 14-19 somites to Protruding-mouth

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biomed. Sci.