FIGURE

Fig. 4

ID

ZDB-FIG-160317-3

Publication

Stumpf et al., 2016 - Differential Requirement for Pten Lipid and Protein Phosphatase Activity during Zebrafish Embryonic Development

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Fig. 4

Pten lipid phosphatase activity is required to rescue the hyperbranching vasculature phenotype observed in Pten double homozygous zebrafish embryos at 3dpf.

Zebrafish embryos from a Tg(kdrl:eGFP) ptena+/-ptenb-/- incross were microinjected at the one-cell stage with 300 pg synthetic mRNA encoding Ptenb-mCherry WT (not shown here, see Fig 3) or with either Ptenb-mcherry C124S, Ptenb-mcherry Y138L, Ptenb-mCherry G129E or with 150 pg of each, Ptenb-eGFP G129E and Ptenb-mCherry Y138L. At 3dpf, the embryos were analyzed for the hyperbranching vessel phenotype by confocal live imaging on a Leica TCS-SPE microscope (anterior to the left, 20x objective, 2µm z-stacks). Pictures show the trunk region distal from the urogenital opening of representative genotyped embryos. Both Ptenb WT (shown in Fig 3) and Ptenb Y138L rescue the hyperbranching phenotype observed at 3dpf.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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